工具选项丰富准备,incubating, washing, or chemical fixation of samples using kinetic motion. Lab mixers that include orbital shakers, vortexes, rockers, rollers and rotators are all offered in a variety of configurations (capacity, speed, duration, slope and more). These common lab instruments have been a necessary component of basic experimentation and sample preparation for decades; almost as long as chemists have understood the impact of motion.
The diversity of these mixing instruments is second only to the media and containers with which they are used. Thus, one must consider not only the procedure being performed, but also the nature of the samples used. Here we discuss this equipment and their most common applications to assist you in selecting the correct solution.
这些乐器是什么？
Orbital Shakers & Vortexers
MaxQ™ 7000 Water Bath Orbital Shaker from Thermo Scientific
Orbital shakers rotate along two planes parallel to the work surface. Occasionally, manufacturers incorporate a third range of motion, such that they reciprocate. This orbital feature allows for enhanced distribution of low-volume samples. These units are ideal for bacterial cell growth, dissolution studies, and staining of electrophoresis gels and blots. These units typically accommodate microcentrifuge tubes, microplates, and gel trays, in addition to larger Erlenmeyer flasks.
使用更高的速度轨道振手剂进行细菌孵育。这些专门的振动器使细胞处于悬浮液状态，提供最佳的生长条件。对于小规模的细菌生长研究，经常将具有较小足迹的振动剂放置在孵化器中以促进生长。有了较大的细菌培养物，可以使用水浴振动器，同时容纳几个大烧瓶。与价格类似的强制空气孵化器相比，这些水浴可为快速加热和低空间偏差提供更有效的热传热。
基准科学多螺旋™涡流
Vortexers不同人物,他们在移动a circular motion. When tubes come in contact with the pad, the motion is transferred to the liquid, which eventually forms a vortex. They are good for mixing and cell suspension, and offer speed and uniformity versus trying to do it manually. Many also offer temperature control.
Rockers
顾名思义，摇滚歌手的设计是通过轻轻来回摇动来混合。它们通常可以像上述一样容纳相同的管，板和印迹托盘。摇滚歌手的目的是提供极低的搅拌，这与振动者更具侵略性的运动不同。例如，当化学固定组织进行孵育时，它至关重要不是to disrupt the tissue while it is being fixed.
BenchRocker™ 2D Variable-Speed Rocker by Benchmark Scientific
Additionally, rockers are used during blotting applications. For Western blot protein analysis, fragile membranes need careful handling, or you risk having to repeat the experiment. Also when blocking membranes, the slow rocking motion provides very little kinetic activity, allowing your blot to properly adhere. Whether fixing, blocking, or incubating tissues, cells, or blots, a rocker is best suited to your application.
Rotators
实验室旋转器在轴周围提供圆形运动。传统的旋转器沿工作表面的平面排列。然后将此轴用于样品的旋转。最近，制造商已经开始在车轮方向上设计这些旋转器，该旋转器可提供更好的力和样品分散体，因为每个样品的旋转都有360°旋转。柔和的终端运动可提供极好的混合，并最小的样品中断。一些显着的应用包括将细胞颗粒和将实验室试剂混合到溶液中。
Compact Digital Waving Rotator by Thermo Scientific
滚筒
管辊设计用于平均收集或圆锥形管的混合。为了获得最佳诊断结果，需要将样品与添加到或衬里的防腐剂或稳定剂充分混合。这些滚筒旨在将样品管固定在固定的水平位置，以在转子之间旋转。它们非常适合临床实验室，医学研究和医师办公室，那里有溢出管的风险可能是危险的。
TubeRoller Tilting Roller (for bottles and tubes) by Benchmark Scientific
最佳选择？
Like other equipment you bring into your lab, application goals, as well as sample size, type and volume, need to be considered when making choices. You wouldn’t use a high-speed shaker to prepare a blood draw for analysis, for example, as the physical response to motion will likely cause erroneous results. Figure out what direction and speed of motion is ideal for the samples types you process, as well as your desired equipment features. For more information about these kinetic helpers, visitLaboratory Rockers, Rotators and Rollersfrom Laboratory-Equipment.com.